Chemical Synthesis of Modified Oligonucleotides Containing 5′‐Amino‐5′‐Deoxy‐5′‐Hydroxymethylthymidine Residues

Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

Chemical Synthesis of Oligonucleotides

It can fairly be said that synthetic oligonucleotides are the fuel that drives the engine of molecular biology. Nearly every technique in use today in molecular biology employs chemically synthesized DNAs or RNAs. This includes PCR, Real-Time PCR, DNA sequencing, site directed mutagenesis, single-nucleotide polymorphism (SNP) assays, microarrays, and the rapidly expanding world of small RNAs. U...

synthesis of binuclear platinum complexes containing biphosphine ligand

هدف از این مطالعه طراحی یک روش کلی برای سنتز یک سری از کمپلکس های دو هسته ای متقارن سیکلومتاله شده پلاتین (ii) بوده که دارای یک لیگاند پل زن(1,2-bis[bis(pentafluoro-phenyl)phosphino]ethane),dfppe, است. از واکنش cis-[pt(r)2(sme2)2]، که در آنr= para-tolyl ، با یک اکی والان از لیگاند benzo[h]quinoline,bhq، در استون محصول [pt(r)(bhq)(sme2)] که در آن r= para-tolyl بدست می آید. همچنین از واکنش 5/0 اک...

Synthesis and properties of modified oligonucleotides.

Phosphorothioate oligonucleotide analogs conjugated to cholesteryl by a neutral, 6 atom linker are more effective inhibitors of HIV-1 in cell culture than the corresponding analogs conjugated via a phosphorothioate group. The antiviral activity correlates with the hydrophobic character of the oligonucleotide. Some new synthetic methodology is also discussed.

Chemical Synthesis and Purification of Oligonucleotides